GoBio Screen
SKU
GoBio™ Screen 7×100 prepacked columns are convenient for fast and easy optimization of methods and parameters, such as selectivity, binding and elution conditions, as well as for small-scale purifications. These columns are prepacked with WorkBeads™ resins which allows quick and easy purification.
Table 1. GoBio Screen 7×100 column characteristics
|
Column characteristics |
|
|
Column hardware |
Acrylic |
|
Top and bottom plugs |
Polypropylene |
|
Top and bottom filters |
Polyamide |
|
Connections |
1/16″ female thread in both ends |
|
Column volume |
3.8 mL |
|
Column dimension |
7 x 100 mm |
| Maximal column hardware pressure1 |
5 bar, 0.5 MPa, 70 psi |
¹ The maximum pressure the packed bed can withstand depends on the sample/liquid viscosity and chromatography resin characteristics. The pressure also depends on the tubing used to connect the column and the system restrictions after the column outlet.
Scale-up
After developing a chromatographic procedure using GoBio Prep 16×100 or GoBio Prep 26×100 column, the column volume can easily be scaled-up by using larger prepacked columns such as, GoBio Prep 16×100 (20 mL), GoBio Prep 26×100 (53 mL) and GoBio Prod 80×200 (1 L). The GoBio Prod prepacked column family is available in column sizes up to 21.4 L.
Table 2. Example of scale-up parameters
|
Column dimension (mm) |
Residence time (minutes) |
Linear flow rate (cm/h) |
Volumetric flow rate (mL/min) |
| 16×100 | 4 | 150 | 5.0 |
| 26×100 | 4 | 150 | 13.3 |
| 80×200 | 8 | 150 | 126 |
| 130×200 | 8 | 150 | 332 |
| 200×200 | 8 | 150 | 785 |
| 240×200 | 8 | 150 | 1131 |
| 330×250 | 10 | 150 | 2138 |

