GoBio Screen

SKU

GoBio™ Screen 7×100 prepacked columns are convenient for fast and easy optimization of methods and parameters, such as selectivity, binding and elution conditions, as well as for small-scale purifications. These columns are prepacked with WorkBeads™ resins which allows quick and easy purification.

Table 1. GoBio Screen 7×100 column characteristics

Column characteristics

Column hardware

Acrylic

Top and bottom plugs

Polypropylene

Top and bottom filters

Polyamide

Connections

1/16″ female thread in both ends

Column volume

3.8 mL

Column dimension

7 x 100 mm
Maximal column hardware pressure1

5 bar, 0.5 MPa, 70 psi

¹ The maximum pressure the packed bed can withstand depends on the sample/liquid viscosity and chromatography resin characteristics. The pressure also depends on the tubing used to connect the column and the system restrictions after the column outlet.

Scale-up
After developing a chromatographic procedure using GoBio Prep 16×100 or GoBio Prep 26×100 column, the column volume can easily be scaled-up by using larger prepacked columns such as, GoBio Prep 16×100 (20 mL), GoBio Prep 26×100 (53 mL) and GoBio Prod 80×200 (1 L). The GoBio Prod prepacked column family is available in column sizes up to 21.4 L.

Table 2. Example of scale-up parameters

Column dimension (mm)

Residence time (minutes)

Linear flow rate (cm/h)

Volumetric flow rate (mL/min)

16×100 4 150 5.0
26×100 4 150 13.3
80×200 8 150 126
130×200 8 150 332
200×200 8 150 785
240×200 8 150 1131
330×250 10 150 2138