Protection of protein A resins during mAb purifications
Today, monoclonal antibodies (mAbs) represent the largest and most progressive biopharmaceutical market. The mAbs used [...]
Effects of elution conditions in the purification of oligonucleotides
Introduction Oligonucleotide synthesis provides custom-made oligonucleotides. The purity of oligonucleotide preparations produced by solid-phase synthesis [...]
Purification of oligonucleotides by anion exchange chromatography
Solid-phase synthesis of oligonucleotides generally give material of rather high purity. However, for many [...]
Conversion of ion-pairing agents/counter ions for oligonucleotides using AIEX
Introduction Efficient purification of therapeutic molecules is of the utmost importance. Equally important, is the [...]
A comparative study of ion exchange vs. ion pair chromatography for preparative separation of oligonucleotides (2025)
The Study: A Comparative Analysis Our study, published in the Journal of Chromatography A, focused [...]
Important optimization parameters for AIEX purifications of 45 nt-long RNA molecules
Introduction RNA molecules have emerged as important molecules in therapeutics, primarily in the form of [...]
Optimization and Scale-up of Oligonucleotide Purifications
Introduction Solid-phase synthesis of oligonucleotides generally gives material of rather high purity. However, for many [...]
Optimizing your IMAC purification
Results from IMAC purifications depend both on the chelated metal ion and the chelator itself [...]
Addressing the challenge of optimizing His-tagged protein purifications
Introduction Immobilized Metal Affinity Chromatography (IMAC) is the commonest technique for purification of His-tagged proteins. [...]
